"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years."
--Pharmaceutical Research

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

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A detailed introduction to the biology and methodology of epithelial cell culture, with most of the main epithelial cell types covered in separate chapters, each with reagents, media and step-wise protocols, and a supplementary chapter reviewing methods and presenting some protocols for the culture of less frequently used epithelia.

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